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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]
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  1. Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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5 October 2016

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lates'07:20, 13 February 2017Thumbnail fi di version fram 07:20, 13 February 20171,020 × 803 (246 KB)Evolution and evolvabilitytext as paths to avoid artefacts
07:19, 13 February 2017Thumbnail fi di version fram 07:19, 13 February 20171,020 × 803 (90 KB)Evolution and evolvabilitytext as paths to avoid artefacts
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